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Journal of Virology, August 2008, p. 7812-7817, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.00561-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Properties of Subviral Particles of Hepatitis B Virus{triangledown}

Ning Chai, Ho Eun Chang, Emmanuelle Nicolas, Ziying Han, Michal Jarnik, and John Taylor*

Fox Chase Cancer Center, Philadelphia, Pennsylvania

Received 13 March 2008/ Accepted 23 May 2008

In the sera of patients infected with hepatitis B virus (HBV), in addition to infectious particles, there is an excess (typically 1,000- to 100,000-fold) of empty subviral particles (SVP) composed solely of HBV envelope proteins in the form of relatively smaller spheres and filaments of variable length. Hepatitis delta virus (HDV) assembly also uses the envelope proteins of HBV to produce an infectious particle. Rate-zonal sedimentation was used to study the particles released from liver cell lines that produced SVP only, HDV plus SVP, and HBV plus SVP. The SVP made in the absence of HBV or HDV were further examined by electron microscopy. They bound efficiently to heparin columns, consistent with an ability to bind cell surface glycosaminoglycans. However, unlike soluble forms of HBV envelope protein that were potent inhibitors, the SVP did not inhibit the ability of HBV and HDV to infect primary human hepatocytes.

* Corresponding author. Mailing address: Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111-2497. Phone: (215) 728-2436. Fax: (215) 728-3105. E-mail: john.taylor{at}fccc.edu

{triangledown} Published ahead of print on 4 June 2008.

Journal of Virology, August 2008, p. 7812-7817, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.00561-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Le Duff, Y., Blanchet, M., Sureau, C. (2009). The Pre-S1 and Antigenic Loop Infectivity Determinants of the Hepatitis B Virus Envelope Proteins Are Functionally Independent. J. Virol. 83: 12443-12451 [Abstract] [Full Text]  
  • Garcia, T., Li, J., Sureau, C., Ito, K., Qin, Y., Wands, J., Tong, S. (2009). Drastic Reduction in the Production of Subviral Particles Does Not Impair Hepatitis B Virus Virion Secretion. J. Virol. 83: 11152-11165 [Abstract] [Full Text]  
  • Salisse, J., Sureau, C. (2009). A Function Essential to Viral Entry Underlies the Hepatitis B Virus "a" Determinant. J. Virol. 83: 9321-9328 [Abstract] [Full Text]  


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