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Journal of Virology, December 2005, p. 15323-15330, Vol. 79, No. 24
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.24.15323-15330.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genomic Sequence Analysis of Epstein-Barr Virus Strain GD1 from a Nasopharyngeal Carcinoma Patient{dagger}

Mu-Sheng Zeng,1,2 Da-Jiang Li,1,2 Qing-Lun Liu,1,2 Li-Bing Song,1,2 Man-Zhi Li,1,2 Ru-Hua Zhang,1,2 Xing-Juan Yu,1,2 Hui-Min Wang,1,2 Ingemar Ernberg,3 and Yi-Xin Zeng1,2*

State Key Laboratory of Oncology in Southern China,1 Departments of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou 510060, People's Republic of China,2 Microbiology and Tumor Biology Center, Karolinska Institutet, Box 280, S-171 77, Stockholm, Sweden3

Received 2 June 2005/ Accepted 22 September 2005

To date, the only entire Epstein-Barr virus (EBV) genomic sequence available in the database is the prototype B95.8, which was derived from an individual with infectious mononucleosis. A causative link between EBV and nasopharyngeal carcinoma (NPC), a disease with a distinctly high incidence in southern China, has been widely investigated. However, no full-length analysis of any substrain of EBV from this area has been reported. In this study, we analyzed the entire genomic sequence of an EBV strain from a patient with NPC in Guangdong, China. This EBV strain was termed GD1 (Guangdong strain 1), and the full-length sequence of GD1 was submitted to the GenBank database. The assigned accession number is AY961628. The entire GD1 sequence is 171,656 bp in length, with 59.5% G+C content and 40.5% A+T content. We detected many sequence variations in GD1 compared to prototypical strain B95.8, including 43 deletion sites, 44 insertion sites, and 1,413 point mutations. Furthermore, we evaluated the frequency of some of these GD1 mutations in Cantonese NPC patients and found them to be highly prevalent. These findings suggest that GD1 is highly representative of the EBV strains isolated from NPC patients in Guangdong, China, an area with the highest incidence of NPC in the world. Furthermore, these findings provide the second full-length sequence analysis of any EBV strain as well as the first full-length sequence analysis of an NPC-derived EBV strain.

* Corresponding author. Mailing address: Department of Experimental Research, Cancer Center, Sun Yat-sen University, 651 Dong-Feng Road East, Guangzhou 510060, China. Phone: 86-20-87343333. Fax: 86-20-87343295. E-mail: yxzeng2005{at}163.com.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, December 2005, p. 15323-15330, Vol. 79, No. 24
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.24.15323-15330.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Xue, S.-A., Griffin, B. E. (2007). Complexities associated with expression of Epstein-Barr virus (EBV) lytic origins of DNA replication. Nucleic Acids Res 35: 3391-3406 [Abstract] [Full Text]  


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