This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Akari, H.
Right arrow Articles by Adachi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Akari, H.
Right arrow Articles by Adachi, A.

 Previous Article  |  Next Article 

Journal of Virology, March 2000, p. 2907-2912, Vol. 74, No. 6
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Nef-Induced Major Histocompatibility Complex Class I Down-Regulation Is Functionally Dissociated from Its Virion Incorporation, Enhancement of Viral Infectivity, and CD4 Down-Regulation

Hirofumi Akari,1,* Stefan Arold,1,dagger Tomoharu Fukumori,1 Toshiyuki Okazaki,1 Klaus Strebel,2 and Akio Adachi1

Department of Virology, The University of Tokushima School of Medicine, Tokushima, Tokushima 770-8503, Japan,1 and Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-04402

Received 1 June 1999/Accepted 2 December 1999

The N-terminal alpha-helix domain of the human immunodeficiency virus type 1 (HIV-1) Nef protein plays important roles in enhancement of viral infectivity, virion incorporation of Nef, and the down-regulation of major histocompatibility complex class I (MHC-I) expression on cell surfaces. In this study, we demonstrated that Met 20 in the alpha-helix domain was indispensable for the ability of Nef to modulate MHC-I expression but not for other events. We also showed that Met 20 was unnecessary for the down-regulation of CD4. These findings indicate that the region governing MHC-I down-regulation is proximate in the alpha-helix domain but is dissociated functionally from that determining enhancement of viral infectivity, virion incorporation of Nef, and CD4 down-regulation.

* Corresponding author. Mailing address: Department of Virology, The University of Tokushima School of Medicine, 3 Kuramoto, Tokushima, Tokushima 770-8503, Japan. Phone: 81-88-633-7079. Fax: 81-88-633-7080. E-mail: akari{at}basic.med.tokushima-u.ac.jp.

dagger Present address: Laboratory of Molecular Biophysics, Oxford, United Kingdom.

Journal of Virology, March 2000, p. 2907-2912, Vol. 74, No. 6
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Baugh, L. L., Garcia, J. V., Foster, J. L. (2008). Functional Characterization of the Human Immunodeficiency Virus Type 1 Nef Acidic Domain. J. Virol. 82: 9657-9667 [Abstract] [Full Text]  
  • Atkins, K. M., Thomas, L., Youker, R. T., Harriff, M. J., Pissani, F., You, H., Thomas, G. (2008). HIV-1 Nef Binds PACS-2 to Assemble a Multikinase Cascade That Triggers Major Histocompatibility Complex Class I (MHC-I) Down-regulation: ANALYSIS USING SHORT INTERFERING RNA AND KNOCK-OUT MICE. J. Biol. Chem. 283: 11772-11784 [Abstract] [Full Text]  
  • DeGottardi, M. Q., Specht, A., Metcalf, B., Kaur, A., Kirchhoff, F., Evans, D. T. (2008). Selective Downregulation of Rhesus Macaque and Sooty Mangabey Major Histocompatibility Complex Class I Molecules by Nef Alleles of Simian Immunodeficiency Virus and Human Immunodeficiency Virus Type 2. J. Virol. 82: 3139-3146 [Abstract] [Full Text]  
  • Lewis, M. J., Balamurugan, A., Ohno, A., Kilpatrick, S., Ng, H. L., Yang, O. O. (2008). Functional Adaptation of Nef to the Immune Milieu of HIV-1 Infection In Vivo. J. Immunol. 180: 4075-4081 [Abstract] [Full Text]  
  • Noviello, C. M., Benichou, S., Guatelli, J. C. (2008). Cooperative Binding of the Class I Major Histocompatibility Complex Cytoplasmic Domain and Human Immunodeficiency Virus Type 1 Nef to the Endosomal AP-1 Complex via Its {micro} Subunit. J. Virol. 82: 1249-1258 [Abstract] [Full Text]  
  • Fujiwara, M., Tanuma, J., Koizumi, H., Kawashima, Y., Honda, K., Mastuoka-Aizawa, S., Dohki, S., Oka, S., Takiguchi, M. (2008). Different Abilities of Escape Mutant-Specific Cytotoxic T Cells To Suppress Replication of Escape Mutant and Wild-Type Human Immunodeficiency Virus Type 1 in New Hosts. J. Virol. 82: 138-147 [Abstract] [Full Text]  
  • Hiyoshi, M., Suzu, S., Yoshidomi, Y., Hassan, R., Harada, H., Sakashita, N., Akari, H., Motoyoshi, K., Okada, S. (2008). Interaction between Hck and HIV-1 Nef negatively regulates cell surface expression of M-CSF receptor. Blood 111: 243-250 [Abstract] [Full Text]  
  • Fujiwara, M., Takiguchi, M. (2007). HIV-1 specific CTLs effectively suppress replication of HIV-1 in HIV-1 infected macrophages. Blood 109: 4832-4838 [Abstract] [Full Text]  
  • Ueno, T., Idegami, Y., Motozono, C., Oka, S., Takiguchi, M. (2007). Altering Effects of Antigenic Variations in HIV-1 on Antiviral Effectiveness of HIV-Specific CTLs. J. Immunol. 178: 5513-5523 [Abstract] [Full Text]  
  • Roeth, J. F., Collins, K. L. (2006). Human Immunodeficiency Virus Type 1 Nef: Adapting to Intracellular Trafficking Pathways. Microbiol. Mol. Biol. Rev. 70: 548-563 [Abstract] [Full Text]  
  • Brenner, M., Munch, J., Schindler, M., Wildum, S., Stolte, N., Stahl-Hennig, C., Fuchs, D., Matz-Rensing, K., Franz, M., Heeney, J., Ten Haaft, P., Swigut, T., Hrecka, K., Skowronski, J., Kirchhoff, F. (2006). Importance of the N-Distal AP-2 Binding Element in Nef for Simian Immunodeficiency Virus Replication and Pathogenicity in Rhesus Macaques. J. Virol. 80: 4469-4481 [Abstract] [Full Text]  
  • O'Neill, E., Kuo, L. S., Krisko, J. F., Tomchick, D. R., Garcia, J. V., Foster, J. L. (2006). Dynamic Evolution of the Human Immunodeficiency Virus Type 1 Pathogenic Factor, Nef. J. Virol. 80: 1311-1320 [Abstract] [Full Text]  
  • Fujiwara, M., Takata, H., Oka, S., Tomiyama, H., Takiguchi, M. (2005). Patterns of Cytokine Production in Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Human CD8+ T Cells after Stimulation with HIV-1-Infected CD4+ T Cells. J. Virol. 79: 12536-12543 [Abstract] [Full Text]  
  • Brown, A., Gartner, S., Kawano, T., Benoit, N., Cheng-Mayer, C. (2005). HLA-A2 down-regulation on primary human macrophages infected with an M-tropic EGFP-tagged HIV-1 reporter virus. J. Leukoc. Biol. 78: 675-685 [Abstract] [Full Text]  
  • Williams, M., Roeth, J. F., Kasper, M. R., Filzen, T. M., Collins, K. L. (2005). Human Immunodeficiency Virus Type 1 Nef Domains Required for Disruption of Major Histocompatibility Complex Class I Trafficking Are Also Necessary for Coprecipitation of Nef with HLA-A2. J. Virol. 79: 632-636 [Abstract] [Full Text]  
  • Tomiyama, H., Fujiwara, M., Oka, S., Takiguchi, M. (2005). Cutting Edge: Epitope-Dependent Effect of Nef-Mediated HLA Class I Down-Regulation on Ability of HIV-1-Specific CTLs to Suppress HIV-1 Replication. J. Immunol. 174: 36-40 [Abstract] [Full Text]  
  • Schindler, M., Munch, J., Brenner, M., Stahl-Hennig, C., Skowronski, J., Kirchhoff, F. (2004). Comprehensive Analysis of Nef Functions Selected in Simian Immunodeficiency Virus-Infected Macaques. J. Virol. 78: 10588-10597 [Abstract] [Full Text]  
  • Larsen, J. E., Massol, R. H., Nieland, T. J. F., Kirchhausen, T. (2004). HIV Nef-mediated Major Histocompatibility Complex Class I Down-Modulation Is Independent of Arf6 Activity. Mol. Biol. Cell 15: 323-331 [Abstract] [Full Text]  
  • Fujita, M., Sakurai, A., Yoshida, A., Miyaura, M., Koyama, A. H., Sakai, K., Adachi, A. (2002). Amino Acid Residues 88 and 89 in the Central Hydrophilic Region of Human Immunodeficiency Virus Type 1 Vif Are Critical for Viral Infectivity by Enhancing the Steady-State Expression of Vif. J. Virol. 77: 1626-1632 [Abstract] [Full Text]  
  • Tomiyama, H., Akari, H., Adachi, A., Takiguchi, M. (2002). Different Effects of Nef-Mediated HLA Class I Down-Regulation on Human Immunodeficiency Virus Type 1-Specific CD8+ T-Cell Cytolytic Activity and Cytokine Production. J. Virol. 76: 7535-7543 [Abstract] [Full Text]  
  • Patel, P. G., Yu Kimata, M. T., Biggins, J. E., Wilson, J. M., Kimata, J. T. (2002). Highly Pathogenic Simian Immunodeficiency Virus mne Variants That Emerge during the Course of Infection Evolve Enhanced Infectivity and the Ability To Downregulate CD4 but Not Class I Major Histocompatibility Complex Antigens. J. Virol. 76: 6425-6434 [Abstract] [Full Text]  
  • Khan, M., Garcia-Barrio, M., Powell, M. D. (2001). Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription. J. Virol. 75: 12081-12087 [Abstract] [Full Text]  
  • Fujita, M., Yoshida, A., Miyaura, M., Sakurai, A., Akari, H., Koyama, A. H., Adachi, A. (2001). Cyclophilin A-Independent Replication of a Human Immunodeficiency Virus Type 1 Isolate Carrying a Small Portion of the Simian Immunodeficiency Virus SIVMAC gag Capsid Region. J. Virol. 75: 10527-10531 [Abstract] [Full Text]  
  • Munch, J., Stolte, N., Fuchs, D., Stahl-Hennig, C., Kirchhoff, F. (2001). Efficient Class I Major Histocompatibility Complex Down-Regulation by Simian Immunodeficiency Virus Nef Is Associated with a Strong Selective Advantage in Infected Rhesus Macaques. J. Virol. 75: 10532-10536 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»