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Journal of Virology, November 2000, p. 10207-10211, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Impairment of Human Immunodeficiency Virus Type 1 (HIV-1) Entry into Jurkat T Cells by Constitutive Expression of the HIV-1 Vpr Protein: Role of CD4 Down-Modulation

Lucia Conti,1 Barbara Varano,1 M. Cristina Gauzzi,1 Paola Matarrese,2 Maurizio Federico,1 Walter Malorni,2 Filippo Belardelli,1 and Sandra Gessani1,*

Laboratories of Virology1 and Ultrastructures,2 Istituto Superiore di Sanità, 00161 Rome, Italy

Received 28 December 1999/Accepted 21 July 2000

Jurkat T-cell clones, stably expressing the human immunodeficiency virus type 1 (HIV-1) Vpr protein, exhibited an impaired susceptibility to HIV-1 infection. A marked down-modulation of surface CD4 receptors was detected in Vpr-expressing clones with respect to control cells. Likewise, a reduced CD4 expression was also observed in parental Jurkat cells infected with wild-type but not with Vpr-mutant HIV-1. Notably, Vpr-expressing clones were fully susceptible to infection with a vesicular stomatitis virus G protein-pseudotyped HIV-1 virus, indicating that a block at the level of viral entry was responsible for the inhibition of viral replication. The effect exerted by Vpr on HIV replication and CD4 expression suggests that this protein can regulate both the establishment of a productive HIV-1 infection and CD4-mediated T-cell functions.

* Corresponding author. Mailing address: Laboratory of Virology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: (39 06) 49903169. Fax: (39 06) 49902097 or 49387184. E-mail: gessani{at}iss.it.

Journal of Virology, November 2000, p. 10207-10211, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Arganaraz, E., Cortes, M. J., Leibel, S., Lama, J. (2002). Human Immunodeficiency Virus Type 1 Vpr Protein Does Not Modulate Surface Expression of the CD4 Receptor. J. Virol. 76: 4125-4130 [Abstract] [Full Text]  
  • Huang, L.-M., Chao, M.-F., Chen, M.-Y., Shih, H.-m., Chiang, Y.-P., Chuang, C.-Y., Lee, C.-Y. (2001). Reciprocal Regulatory Interaction between Human Herpesvirus 8 and Human Immunodeficiency Virus Type 1. J. Biol. Chem. 276: 13427-13432 [Abstract] [Full Text]  


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