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Journal of Virology, May 2000, p. 4541-4548, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identifying the Determinants in the Equatorial Domain of Buchnera GroEL Implicated in Binding Potato Leafroll Virus

Saskia A. Hogenhout,1 Frank van der Wilk,1 Martin Verbeek,1 Rob W. Goldbach,2 and Johannes F. J. M. van den Heuvel1,*

Plant Research International, 6700 AA Wageningen,1 and Wageningen University, 6709 PD Wageningen,2 The Netherlands

Received 9 September 1999/Accepted 22 February 2000

Luteoviruses avoid degradation in the hemolymph of their aphid vector by interacting with a GroEL homolog from the aphid's primary endosymbiotic bacterium (Buchnera sp.). Mutational analysis of GroEL from the primary endosymbiont of Myzus persicae (MpB GroEL) revealed that the amino acids mediating binding of Potato leafroll virus (PLRV; Luteoviridae) are located within residues 9 to 19 and 427 to 457 of the N-terminal and C-terminal regions, respectively, of the discontinuous equatorial domain. Virus overlay assays with a series of overlapping synthetic decameric peptides and their derivatives demonstrated that R13, K15, L17, and R18 of the N-terminal region and R441 and R445 of the C-terminal region of the equatorial domain of GroEL are critical for PLRV binding. Replacement of R441 and R445 by alanine in full-length MpB GroEL and in MpB GroEL deletion mutants reduced but did not abolish PLRV binding. Alanine substitution of either R13 or K15 eliminated the PLRV-binding capacity of the other and those of L17 and R18. In the predicted tertiary structure of GroEL, the determinants mediating virus binding are juxtaposed in the equatorial plain.

* Corresponding author. Mailing address: Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands. Phone: 31 317 476141. Fax: 31 317 410113. E-mail: J.F.J.M.vandenHeuvel{at}plant.wag-ur.nl.

Journal of Virology, May 2000, p. 4541-4548, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Peter, K. A., Liang, D., Palukaitis, P., Gray, S. M. (2008). Small deletions in the potato leafroll virus readthrough protein affect particle morphology, aphid transmission, virus movement and accumulation. J. Gen. Virol. 89: 2037-2045 [Abstract] [Full Text]  
  • Hohn, T. (2007). Plant virus transmission from the insect point of view. Proc. Natl. Acad. Sci. USA 104: 17905-17906 [Full Text]  
  • Fares, M. A., Barrio, E., Sabater-Munoz, B., Moya, A. (2002). The Evolution of the Heat-Shock Protein GroEL from Buchnera, the Primary Endosymbiont of Aphids, Is Governed by Positive Selection. Mol Biol Evol 19: 1162-1170 [Abstract] [Full Text]  
  • Scholthof, H. B. (2001). Molecular Plant-Microbe Interactions That Cut the Mustard. Plant Physiol. 127: 1476-1483 [Full Text]  


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