This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yuan, L.
Right arrow Articles by Saif, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yuan, L.
Right arrow Articles by Saif, L. J.

 Previous Article  |  Next Article 

J. Virol., Jan 1998, 330-338, Vol 72, No. 1
Copyright © 1998, American Society for Microbiology

Antibody-secreting cell responses and protective immunity assessed in gnotobiotic pigs inoculated orally or intramuscularly with inactivated human rotavirus

L Yuan, SY Kang, LA Ward, TL To and LJ Saif
Department of Veterinary Preventive Medicine, Ohio Agriculture Research and Development Center, The Ohio State University, Wooster 44691-4096, USA.

Newborn gnotobiotic pigs were inoculated twice perorally (p.o.) (group 1) or intramuscularly (i.m.) (group 2) or three times i.m. (group 3) with inactivated Wa strain human rotavirus and challenged with virulent Wa human rotavirus 20 to 24 days later. To assess correlates of protection, antibody-secreting cells (ASC) were enumerated in intestinal and systemic lymphoid tissues from pigs in each group at selected postinoculation days (PID) or postchallenge days. Few virus- specific ASC were detected in any tissues of group 1 pigs prior to challenge. By comparison, groups 2 and 3 had significantly greater numbers of virus-specific immunoglobulin M (IgM) ASC in intestinal and splenic tissues at PID 8 and significantly greater numbers of virus- specific IgG ASC and IgG memory B cells in spleen and blood at challenge. However, as for group 1, few virus-specific IgA ASC or IgA memory B cells were detected in any tissues of group 2 and 3 pigs. Neither p.o. nor i.m. inoculation conferred significant protection against virulent Wa rotavirus challenge (0 to 6% protection rate), and all groups showed significant anamnestic virus-specific IgG and IgA ASC responses. Hence, high numbers of IgG ASC or memory IgG ASC in the systemic lymphoid tissues at the time of challenge did not correlate with protection. Further, our findings suggest that inactivated Wa human rotavirus administered either p.o. or parenterally is significantly less effective in inducing intestinal IgA ASC responses and conferring protective immunity than live Wa human rotavirus inoculated orally, as reported earlier (L. Yuan, L. A. Ward, B. I. Rosen, T. L. To, and L. J. Saif, J. Virol. 70:3075-3083, 1996). Thus, more efficient mucosal delivery systems and rotavirus vaccination strategies are needed to induce intestinal IgA ASC responses, identified previously as a correlate of protective immunity to rotavirus.


This article has been cited by other articles:

  • Souza, M., Azevedo, M. S. P., Jung, K., Cheetham, S., Saif, L. J. (2008). Pathogenesis and Immune Responses in Gnotobiotic Calves after Infection with the Genogroup II.4-HS66 Strain of Human Norovirus. J. Virol. 82: 1777-1786 [Abstract] [Full Text]  
  • Souza, M., Cheetham, S. M., Azevedo, M. S. P., Costantini, V., Saif, L. J. (2007). Cytokine and Antibody Responses in Gnotobiotic Pigs after Infection with Human Norovirus Genogroup II.4 (HS66 Strain). J. Virol. 81: 9183-9192 [Abstract] [Full Text]  
  • Dvorak, C. M. T., Hirsch, G. N., Hyland, K. A., Hendrickson, J. A., Thompson, B. S., Rutherford, M. S., Murtaugh, M. P. (2006). Genomic dissection of mucosal immunobiology in the porcine small intestine. Physiol. Genomics 28: 5-14 [Abstract] [Full Text]  
  • Azevedo, M. S. P., Yuan, L., Pouly, S., Gonzales, A. M., Jeong, K. I., Nguyen, T. V., Saif, L. J. (2006). Cytokine Responses in Gnotobiotic Pigs after Infection with Virulent or Attenuated Human Rotavirus. J. Virol. 80: 372-382 [Abstract] [Full Text]  
  • Lindesmith, L., Moe, C., LePendu, J., Frelinger, J. A., Treanor, J., Baric, R. S. (2005). Cellular and Humoral Immunity following Snow Mountain Virus Challenge. J. Virol. 79: 2900-2909 [Abstract] [Full Text]  
  • Azevedo, M. S. P., Yuan, L., Iosef, C., Chang, K.-O., Kim, Y., Van Nguyen, T., Saif, L. J. (2004). Magnitude of Serum and Intestinal Antibody Responses Induced by Sequential Replicating and Nonreplicating Rotavirus Vaccines in Gnotobiotic Pigs and Correlation with Protection. CVI 11: 12-20 [Abstract] [Full Text]  
  • Yuan, L., Iosef, C., Azevedo, M. S. P., Kim, Y., Qian, Y., Geyer, A., Nguyen, T. V., Chang, K.-O., Saif, L. J. (2001). Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with Mutant Escherichia coli Heat-Labile Toxin in Gnotobiotic Pigs. J. Virol. 75: 9229-9238 [Abstract] [Full Text]  
  • Chang, K. O., Vandal, O. H., Yuan, L., Hodgins, D. C., Saif, L. J. (2001). Antibody-Secreting Cell Responses to Rotavirus Proteins in Gnotobiotic Pigs Inoculated with Attenuated or Virulent Human Rotavirus. J. Clin. Microbiol. 39: 2807-2813 [Abstract] [Full Text]  
  • Yuan, L., Geyer, A., Hodgins, D. C., Fan, Z., Qian, Y., Chang, K.-O., Crawford, S. E., Parreño, V., Ward, L. A., Estes, M. K., Conner, M. E., Saif, L. J. (2000). Intranasal Administration of 2/6-Rotavirus-Like Particles with Mutant Escherichia coli Heat-Labile Toxin (LT-R192G) Induces Antibody-Secreting Cell Responses but Not Protective Immunity in Gnotobiotic Pigs. J. Virol. 74: 8843-8853 [Abstract] [Full Text]  
  • Hodgins, D. C., Kang, S. Y., deArriba, L., Parreno, V., Ward, L. A., Yuan, L., To, T., Saif, L. J. (1999). Effects of Maternal Antibodies on Protection and Development of Antibody Responses to Human Rotavirus in Gnotobiotic Pigs. J. Virol. 73: 186-197 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»