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J. Virol. doi:10.1128/JVI.01086-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Glutamine deprivation causes enhanced plating efficiency of a herpes simplex virus type 1 ICP0-null mutant

Departments of Medicine and Microbiology and Molecular Genetics, Harvard Medical School at the Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215; and Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003

^* To whom correspondence should be addressed. Email: rbring{at}email.arizona.edu.

	Abstract

Isoleucine deprivation of cellular monolayers prior to infection has been reported to result in partial complementation of an HSV-1 ICP0 null-mutant. We now report that glutamine deprivation alone is able to enhance the plating efficiency of an ICP0^- virus, and isoleucine deprivation has little or no effect. Because low glutamine is associated with stress and because stress is known to induce reactivation, low levels Gln may be relevant to the reactivation of HSV-1 from latency. Additionally, we demonstrate that arginine and methionine deprivation result in partial complementation of the ICP0^- virus.