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Journal of Virology, February 2008, p. 1118-1127, Vol. 82, No. 3
0022-538X/08/$08.00+0     doi:10.1128/JVI.01758-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II{triangledown}

Jinhong Chang,{ddagger},{dagger} Xingcao Nie,{dagger} Ho Eun Chang, Ziying Han, and John Taylor*

Fox Chase Cancer Center, Philadelphia, Pennsylvania

Received 10 August 2007/ Accepted 6 November 2007

Previous studies have indicated that the replication of the RNA genome of hepatitis delta virus (HDV) involves redirection of RNA polymerase II (Pol II), a host enzyme that normally uses DNA as a template. However, there has been some controversy about whether in one part of this HDV RNA transcription, a polymerase other than Pol II is involved. The present study applied a recently described cell system (293-HDV) of tetracycline-inducible HDV RNA replication to provide new data regarding the involvement of host polymerases in HDV transcription. The data generated with a nuclear run-on assay demonstrated that synthesis not only of genomic RNA but also of its complement, the antigenome, could be inhibited by low concentrations of amanitin specific for Pol II transcription. Subsequent studies used immunoprecipitation and rate-zonal sedimentation of nuclear extracts together with double immunostaining of 293-HDV cells, in order to examine the associations between Pol II and HDV RNAs, as well as the small delta antigen, an HDV-encoded protein known to be essential for replication. Findings include evidence that HDV replication is somehow able to direct the available delta antigen to sites in the nucleoplasm, almost exclusively colocalized with Pol II in what others have described as transcription factories.

* Corresponding author. Mailing address: Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111-2497. Phone: (215) 728-2436. Fax: (215) 728-3105. E-mail: john.taylor{at}fccc.edu

{triangledown} Published ahead of print on 21 November 2007.

{ddagger} Present address: Drexel Institute for Biotechnology and Virology Research, 3805 Old Easton Road, Doylestown, PA 18902.

{dagger} J.C. and X.N. contributed equally to this study.

Journal of Virology, February 2008, p. 1118-1127, Vol. 82, No. 3
0022-538X/08/$08.00+0     doi:10.1128/JVI.01758-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Han, Z., Alves, C., Gudima, S., Taylor, J. (2009). Intracellular Localization of Hepatitis Delta Virus Proteins in the Presence and Absence of Viral RNA Accumulation. J. Virol. 83: 6457-6463 [Abstract] [Full Text]  
  • Chen, Y.-S., Huang, W.-H., Hong, S.-Y., Tsay, Y.-G., Chen, P.-J. (2008). ERK1/2-Mediated Phosphorylation of Small Hepatitis Delta Antigen at Serine 177 Enhances Hepatitis Delta Virus Antigenomic RNA Replication. J. Virol. 82: 9345-9358 [Abstract] [Full Text]  
  • Tseng, C.-H., Jeng, K.-S., Lai, M. M. C. (2008). Transcription of Subgenomic mRNA of Hepatitis Delta Virus Requires a Modified Hepatitis Delta Antigen That Is Distinct from Antigenomic RNA Synthesis. J. Virol. 82: 9409-9416 [Abstract] [Full Text]  


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