This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Turville, S. G.
Right arrow Articles by Schols, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Turville, S. G.
Right arrow Articles by Schols, D.

 Previous Article  |  Next Article 

Journal of Virology, November 2005, p. 13519-13527, Vol. 79, No. 21
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.21.13519-13527.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Sugar-Binding Proteins Potently Inhibit Dendritic Cell Human Immunodeficiency Virus Type 1 (HIV-1) Infection and Dendritic-Cell-Directed HIV-1 Transfer

Stuart G. Turville,{dagger} Kurt Vermeire, Jan Balzarini, and Dominique Schols*

Rega Institute for Medical Research, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium

Received 28 January 2005/ Accepted 9 August 2005

Both endocytic uptake and viral fusion can lead to human immunodeficiency virus type 1 (HIV-1) transfer to CD4+ lymphocytes, either through directional regurgitation (infectious transfer in trans [I-IT]) or through de novo viral production in dendritic cells (DCs) resulting in a second-phase transfer to CD4+ lymphocytes (infectious second-phase transfer [I-SPT]). We have evaluated in immature monocyte-derived DCs both pathways of transfer with regard to their susceptibilities to being blocked by potential microbicidal compounds, including cyanovirin (CNV); the plant lectins Hippeastrum hybrid agglutinin, Galanthus nivalis agglutinin, Urtica dioica agglutinin, and Cymbidium hybrid agglutinin; and the glycan mannan. I-IT was a relatively inefficient means of viral transfer compared to I-SPT at both high and low levels of the viral inoculum. CNV was able to completely block I-IT at 15 µg/ml. All other compounds except mannan could inhibit I-IT by at least 90% when used at doses of 15 µg/ml. In contrast, efficient inhibition of I-SPT was remarkably harder to achieve, as 50% effective concentration levels for plant lectins and CNV to suppress this mode of HIV-1 transfer increased significantly. Thus, our findings indicate that I-SPT may be more elusive to targeting by antiviral drugs and stress the need for drugs affecting the pronounced inhibition of the infection of DCs by HIV-1.

* Corresponding author. Mailing address: Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium. Phone: 32-16-337373. Fax: 32-16-337340. E-mail: Dominique.schols{at}rega.kuleuven.be.

{dagger} Present address: Center for Biomedical Research, Population Council, New York, NY 10021.

Journal of Virology, November 2005, p. 13519-13527, Vol. 79, No. 21
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.21.13519-13527.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Trapp, S., Derby, N. R., Singer, R., Shaw, A., Williams, V. G., Turville, S. G., Bess, J. W. Jr., Lifson, J. D., Robbiani, M. (2009). Double-Stranded RNA Analog Poly(I:C) Inhibits Human Immunodeficiency Virus Amplification in Dendritic Cells via Type I Interferon-Mediated Activation of APOBEC3G. J. Virol. 83: 884-895 [Abstract] [Full Text]  
  • Buffa, V., Stieh, D., Mamhood, N., Hu, Q., Fletcher, P., Shattock, R. J. (2009). Cyanovirin-N potently inhibits human immunodeficiency virus type 1 infection in cellular and cervical explant models. J. Gen. Virol. 90: 234-243 [Abstract] [Full Text]  
  • Frank, I., Stossel, H., Gettie, A., Turville, S. G., Bess, J. W. Jr., Lifson, J. D., Sivin, I., Romani, N., Robbiani, M. (2008). A Fusion Inhibitor Prevents Spread of Immunodeficiency Viruses, but Not Activation of Virus-Specific T Cells, by Dendritic Cells. J. Virol. 82: 5329-5339 [Abstract] [Full Text]  
  • Sprague, W. S., Robbiani, M., Avery, P. R., O'Halloran, K. P., Hoover, E. A. (2008). Feline immunodeficiency virus dendritic cell infection and transfer. J. Gen. Virol. 89: 709-715 [Abstract] [Full Text]  
  • Saidi, H., Magri, G., Carbonneil, C., Nasreddine, N., Requena, M., Belec, L. (2007). IFN-{gamma}-activated monocytes weakly produce HIV-1 but induce the recruitment of HIV-sensitive T cells and enhance the viral production by these recruited T cells. J. Leukoc. Biol. 81: 642-653 [Abstract] [Full Text]  
  • Balzarini, J., Van Herrewege, Y., Vermeire, K., Vanham, G., Schols, D. (2007). Carbohydrate-Binding Agents Efficiently Prevent Dendritic Cell-Specific Intercellular Adhesion Molecule-3-Grabbing Nonintegrin (DC-SIGN)-Directed HIV-1 Transmission to T Lymphocytes. Mol. Pharmacol. 71: 3-11 [Abstract] [Full Text]  
  • Trapp, S., Turville, S. G., Robbiani, M. (2006). Slamming the door on unwanted guests: why preemptive strikes at the mucosa may be the best strategy against HIV. J. Leukoc. Biol. 80: 1076-1083 [Full Text]  
  • Donaghy, H., Wilkinson, J., Cunningham, A. L. (2006). HIV interactions with dendritic cells: has our focus been too narrow?. J. Leukoc. Biol. 80: 1001-1012 [Abstract] [Full Text]  
  • Saidi, H., Eslaphazir, J., Carbonneil, C., Carthagena, L., Requena, M., Nassreddine, N., Belec, L. (2006). Differential Modulation of Human Lactoferrin Activity against Both R5 and X4-HIV-1 Adsorption on Epithelial Cells and Dendritic Cells by Natural Antibodies. J. Immunol. 177: 5540-5549 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»