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Journal of Virology, July 2005, p. 8651-8654, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8651-8654.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Growth of Respiratory Syncytial Virus in Primary Epithelial Cells from the Human Respiratory Tract

Peter F. Wright,1,2,4* Mine R. Ikizler,1 Ricardo A. Gonzales,1 Kecia N. Carroll,1 Joyce E. Johnson,4 and Jay A. Werkhaven3

Departments of Pediatrics,1 Microbiology and Immunology,2 Otolaryngology,3 Pathology, Vanderbilt University Medical Center, Nashville, Tennessee4

Received 17 November 2004/ Accepted 1 March 2005

Respiratory syncytial virus (RSV) is the most important cause of lower respiratory tract disease in infants and children. To study RSV replication, we have developed an in vitro model of human nasopharyngeal mucosa, human airway epithelium (HAE). RSV grows to moderate titers in HAE, though they are significantly lower than those in a continuous epithelial cell line, HEp-2. In HAE, RSV spreads over time to form focal collections of infected cells causing minimal cytopathic effect. Unlike HEp-2 cells, in which wild-type and live-attenuated vaccine candidate viruses grow equally well, the vaccine candidates exhibit growth in HAE that parallels their level of attenuation in children.

* Corresponding author. Mailing address: Vanderbilt University Medical Center, Department of Pediatrics, D-7235 MCN, 1215 21st Ave., Nashville, TN 37232. Phone: (615) 322-2250. Fax: (615) 343-9723. E-mail: peter.wright{at}vanderbilt.edu.

Journal of Virology, July 2005, p. 8651-8654, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8651-8654.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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