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Journal of Virology, July 2005, p. 8625-8628, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8625-8628.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Real-Time Detection of Virus Particles and Viral Protein Expression with Two-Color Nanoparticle Probes

Amit Agrawal,1 Ralph A. Tripp,2* Larry J. Anderson,3 and Shuming Nie1*

Departments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001,1 Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Respiratory and Enteric Viruses, Atlanta,3 College of Veterinary Medicine, Department of Infectious Diseases, University of Georgia, Athens, Georgia2

Received 3 November 2004/ Accepted 17 February 2005

Respiratory syncytial virus (RSV) mediates serious lower respiratory tract illness in infants and young children and is a significant pathogen of the elderly and immune compromised. Rapid and sensitive RSV diagnosis is important to infection control and efforts to develop antiviral drugs. Current RSV detection methods are limited by sensitivity and/or time required for detection. In this study, we show that antibody-conjugated nanoparticles rapidly and sensitively detect RSV and estimate relative levels of surface protein expression. A major development is use of dual-color quantum dots or fluorescence energy transfer nanobeads that can be simultaneously excited with a single light source.

* Corresponding author. Mailing address for Ralph A. Tripp: College of Veterinary Medicine, Department of Infectious Diseases, University of Georgia, Athens, GA 30602. Phone: (706) 542-1557. Fax: (706) 583-0176. E-mail: ratripp{at}uga.edu. Mailing address for Shuming Nie: Departments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001, Atlanta, GA 30322. Phone: (404) 712-8595. Fax: (404) 727-9873. E-mail: snie{at}emory.edu.

Journal of Virology, July 2005, p. 8625-8628, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8625-8628.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jain, K. K. (2007). Applications of Nanobiotechnology in Clinical Diagnostics. Clin. Chem. 53: 2002-2009 [Abstract] [Full Text]  


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