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Journal of Virology, June 2005, p. 7938-7941, Vol. 79, No. 12
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.12.7938-7941.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

G-Protein Signaling Triggered by R5 Human Immunodeficiency Virus Type 1 Increases Virus Replication Efficiency in Primary T Lymphocytes

Yea-Lih Lin,1 Clément Mettling,1 Pierre Portalès,2 Brigitte Réant,1,2 Jacques Clot,2 and Pierre Corbeau1,2*

Institut de Génétique Humaine, CNRS UPR1142,1 Laboratoire d'Immunologie, Hôpital Saint Eloi, Montpellier, France2

Received 15 March 2004/ Accepted 18 February 2005

The binding of R5 envelope to CCR5 during human immunodeficiency virus type 1 (HIV-1) entry provokes cell activation, which has so far been considered to have no effect on virus replication, since signaling-defective CCR5 molecules have been shown to function normally as HIV-1 coreceptors on transformed cells or mitogen-stimulated T lymphocytes. As the background state of activation of these cells might have biased the results, we performed experiments using the same approach but with nonactivated primary T lymphocytes. We now report that the single R126N mutation in the DRY motif, involved in G-protein coupling, results in a signaling-defective CCR5 coreceptor with a drastically impaired capacity to support HIV-1 infection.

* Corresponding author. Mailing address: Laboratoire d'Immunologie, Hopital Saint Eloi, 80 avenue Augustin Fliche, 34295 Montpellier Cedex 5, France. Phone: 33-4-67-33-71-35. Fax: 33-4-67-33-71-29. E-mail: pierre.corbeau{at}igh.cnrs.fr.

Journal of Virology, June 2005, p. 7938-7941, Vol. 79, No. 12
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.12.7938-7941.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Heredia, A., Gilliam, B., Latinovic, O., Le, N., Bamba, D., DeVico, A., Melikyan, G. B., Gallo, R. C., Redfield, R. R. (2007). Rapamycin Reduces CCR5 Density Levels on CD4 T Cells, and This Effect Results in Potentiation of Enfuvirtide (T-20) against R5 Strains of Human Immunodeficiency Virus Type 1 In Vitro. Antimicrob. Agents Chemother. 51: 2489-2496 [Abstract] [Full Text]  


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