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Journal of Virology, May 2005, p. 6505-6510, Vol. 79, No. 10
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.10.6505-6510.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Human Circovirus TT Virus Genotype 6 Expresses Six Proteins following Transfection of a Full-Length Clone{dagger}

Jianming Qiu,1* Laura Kakkola,2 Fang Cheng,1 Chaoyang Ye,1 Maria Söderlund-Venermo,2 Klaus Hedman,2 and David J. Pintel1

Department of Molecular Microbiology and Immunology, University of Missouri—Columbia, Columbia, Missouri 65212,1 Department of Virology, Haartman Institute and Helsinki University Central Hospital, POB 21, FIN-00014 University of Helsinki, Helsinki, Finland2

Received 13 August 2004/ Accepted 4 January 2005

The expression profile of the circovirus TTV has not yet been fully characterized. In this paper, we show that following transfection of a full-length viral clone of TTV genotype 6, each of the three virally encoded mRNAs is translated from two initiating AUGs, and therefore, the TTV genome generates at least six proteins. Localization studies of hemagglutinin-tagged versions of these proteins in fixed cells, and green fluorescent protein-tagged versions of these proteins in living cells, expressed following transfection, demonstrated that two were primarily nuclear, two were primarily cytoplasmic, and two were found throughout the cell.

* Corresponding author. Mailing address: M 612 Medical Sciences Bldg., Department of Molecular Microbiology and Immunology, University of Missouri—Columbia, School of Medicine, Columbia, MO 65212. Phone: (573) 882-3171. Fax: (573) 882-4287. E-mail: qiuj{at}missouri.edu.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, May 2005, p. 6505-6510, Vol. 79, No. 10
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.10.6505-6510.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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