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Journal of Virology, May 2003, p. 6050-6054, Vol. 77, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.10.6050-6054.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The NB Protein of Influenza B Virus Is Not Necessary for Virus Replication In Vitro

Masato Hatta1 and Yoshihiro Kawaoka1,2,3*

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, Wisconsin 53706,1 Institute of Medical Science, University of Tokyo, Tokyo 108-8639 ,2 Core Research for Evaluational Science and Technology, Japan Science and Technology Corporation, Saitama 332-0012, Japan3

Received 19 November 2002/ Accepted 10 February 2003

The NB protein of influenza B virus is thought to function as an ion channel and therefore would be expected to have an essential function in viral replication. Because direct evidence for its absolute requirement in the viral life cycle is lacking, we generated NB knockout viruses by reverse genetics and tested their growth properties both in vitro and in vivo. Mutants not expressing NB replicated as efficiently as the wild-type virus in cell culture, whereas in mice they showed restricted growth compared with findings for the wild-type virus. Thus, the NB protein is not essential for influenza B virus replication in cell culture but promotes efficient growth in mice.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, WI 53706. Phone: (608) 265-4925. Fax: (608) 265-5622. E-mail: kawaokay{at}svm.vetmed.wisc.edu.

Journal of Virology, May 2003, p. 6050-6054, Vol. 77, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.10.6050-6054.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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