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Journal of Virology, April 2000, p. 3899-3904, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Ultrastructural Organization of Recombinant Marburg Virus Nucleoprotein: Comparison with Marburg Virus Inclusions

Larissa Kolesnikova,1 Elke Mühlberger,2 Elena Ryabchikova,1 and Stephan Becker2,*

State Scientific Research Center of Virology and Biothechnology, Vector Institute of Molecular Biology, Laboratory of Ultrastructure and Pathomorphology, 633159 Koltsovo, Novosibirsk Region, Russia,1 and Institut für Virologie der Philipps-Universität Marburg, D-35037 Marburg, Germany2

Received 30 August 1999/Accepted 20 January 2000

HeLa cells expressing the recombinant Marburg virus (MBGV) nucleoprotein (NP) have been studied by immunoelectron microscopy. It was found that MBGV NPs assembled into large aggregates which were in close association with membranes of the rough endoplasmic reticulum. Further analysis of these aggregates revealed that NPs formed tubule-like structures which were arranged in a hexagonal pattern. A similar pattern of preformed nucleocapsids was detected in intracellular inclusions induced by MBGV infection. Our data indicated that MBGV NP is able to form nucleocapsid-like structures in the absence of the authentic viral genome and other nucleocapsid-associated proteins.

* Corresponding author. Mailing address: Institut für Virologie der Philipps-Universität Marburg, Robert-Koch-Strasse 17, D-35037 Marburg, Germany. Phone: 06421-28-5433. Fax: 06421-28-5482. E-mail: becker{at}mailer.uni-marburg.de.

Journal of Virology, April 2000, p. 3899-3904, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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