This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pho, M. T.
Right arrow Articles by Atwood, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pho, M. T.
Right arrow Articles by Atwood, W. J.

 Previous Article  |  Next Article 

Journal of Virology, March 2000, p. 2288-2292, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

JC Virus Enters Human Glial Cells by Clathrin-Dependent Receptor-Mediated Endocytosis

M. T. Pho, A. Ashok, and Walter J. Atwood*

Department of Molecular Microbiology and Immunology, Brown University, Providence, Rhode Island 02912

Received 4 August 1999/Accepted 10 December 1999

The human polyomavirus JC virus (JCV) is the etiologic agent of a fatal central nervous system (CNS) demyelinating disease known as progressive multifocal leukoencephalopathy (PML). PML occurs predominantly in immunosuppressed patients and has increased dramatically as a result of the AIDS pandemic. The major target cell of JCV infection and lytic replication in the CNS is the oligodendrocyte. The mechanisms by which JCV initiates and establishes infection of these glial cells are not understood. The initial interaction between JCV and glial cells involves virus binding to N-linked glycoproteins containing terminal alpha (2-6)-linked sialic acids. The subsequent steps of entry and targeting of the viral genome to the nucleus have not been described. In this report, we compare the kinetics and mechanisms of infectious entry of JCV into human glial cells with that of the related polyomavirus, simian virus 40 (SV40). We demonstrate that JCV, unlike SV40, enters glial cells by receptor-mediated clathrin-dependent endocytosis.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Immunology, Brown University, Box G-B616, 117 Meeting St., Providence, RI 02912. Phone: (401) 863-3116. Fax: (401) 863-1971. E-mail: Walter_Atwood{at}Brown.edu.

Journal of Virology, March 2000, p. 2288-2292, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Goodwin, E. C., Atwood, W. J., DiMaio, D. (2009). High-Throughput Cell-Based Screen for Chemicals That Inhibit Infection by Simian Virus 40 and Human Polyomaviruses. J. Virol. 83: 5630-5639 [Abstract] [Full Text]  
  • Brickelmaier, M., Lugovskoy, A., Kartikeyan, R., Reviriego-Mendoza, M. M., Allaire, N., Simon, K., Frisque, R. J., Gorelik, L. (2009). Identification and Characterization of Mefloquine Efficacy against JC Virus In Vitro. Antimicrob. Agents Chemother. 53: 1840-1849 [Abstract] [Full Text]  
  • Laniosz, V., Holthusen, K. A., Meneses, P. I. (2008). Bovine Papillomavirus Type 1: from Clathrin to Caveolin. J. Virol. 82: 6288-6298 [Abstract] [Full Text]  
  • Huang, J.-H., Qi, Z., Wu, F., Kotula, L., Jiang, S., Chen, Y.-H. (2008). Interaction of HIV-1 gp41 Core with NPF Motif in Epsin: IMPLICATION IN ENDOCYTOSIS OF HIV. J. Biol. Chem. 283: 14994-15002 [Abstract] [Full Text]  
  • Maginnis, M. S., Mainou, B. A., Derdowski, A., Johnson, E. M., Zent, R., Dermody, T. S. (2008). NPXY Motifs in the {beta}1 Integrin Cytoplasmic Tail Are Required for Functional Reovirus Entry. J. Virol. 82: 3181-3191 [Abstract] [Full Text]  
  • Frampton, A. R. Jr., Stolz, D. B., Uchida, H., Goins, W. F., Cohen, J. B., Glorioso, J. C. (2007). Equine Herpesvirus 1 Enters Cells by Two Different Pathways, and Infection Requires the Activation of the Cellular Kinase ROCK1. J. Virol. 81: 10879-10889 [Abstract] [Full Text]  
  • Smith, J. L., Campos, S. K., Ozbun, M. A. (2007). Human Papillomavirus Type 31 Uses a Caveolin 1- and Dynamin 2-Mediated Entry Pathway for Infection of Human Keratinocytes. J. Virol. 81: 9922-9931 [Abstract] [Full Text]  
  • Moriyama, T., Marquez, J. P., Wakatsuki, T., Sorokin, A. (2007). Caveolar Endocytosis Is Critical for BK Virus Infection of Human Renal Proximal Tubular Epithelial Cells. J. Virol. 81: 8552-8562 [Abstract] [Full Text]  
  • Bohl, D. L., Brennan, D. C. (2007). BK Virus Nephropathy and Kidney Transplantation. CJASN 2: S36-S46 [Abstract] [Full Text]  
  • Forzan, M., Marsh, M., Roy, P. (2007). Bluetongue Virus Entry into Cells. J. Virol. 81: 4819-4827 [Abstract] [Full Text]  
  • Cantin, C., Holguera, J., Ferreira, L., Villar, E., Munoz-Barroso, I. (2007). Newcastle disease virus may enter cells by caveolae-mediated endocytosis. J. Gen. Virol. 88: 559-569 [Abstract] [Full Text]  
  • Berger, J R (2006). Natalizumab and progressive multifocal leucoencephalopathy. Ann Rheum Dis 65: iii48-iii53 [Abstract] [Full Text]  
  • Querbes, W., O'Hara, B. A., Williams, G., Atwood, W. J. (2006). Invasion of Host Cells by JC Virus Identifies a Novel Role for Caveolae in Endosomal Sorting of Noncaveolar Ligands. J. Virol. 80: 9402-9413 [Abstract] [Full Text]  
  • Dang, X., Koralnik, I. J. (2006). A granule cell neuron-associated JC virus variant has a unique deletion in the VP1 gene. J. Gen. Virol. 87: 2533-2537 [Abstract] [Full Text]  
  • Liebl, D., Difato, F., Hornikova, L., Mannova, P., Stokrova, J., Forstova, J. (2006). Mouse Polyomavirus Enters Early Endosomes, Requires Their Acidic pH for Productive Infection, and Meets Transferrin Cargo in Rab11-Positive Endosomes. J. Virol. 80: 4610-4622 [Abstract] [Full Text]  
  • Sariyer, I. K., Akan, I., Palermo, V., Gordon, J., Khalili, K., Safak, M. (2006). Phosphorylation Mutants of JC Virus Agnoprotein Are Unable To Sustain the Viral Infection Cycle.. J. Virol. 80: 3893-3903 [Abstract] [Full Text]  
  • Low, J. A., Magnuson, B., Tsai, B., Imperiale, M. J. (2006). Identification of Gangliosides GD1b and GT1b as Receptors for BK Virus. J. Virol. 80: 1361-1366 [Abstract] [Full Text]  
  • Eash, S., Atwood, W. J. (2005). Involvement of Cytoskeletal Components in BK Virus Infectious Entry. J. Virol. 79: 11734-11741 [Abstract] [Full Text]  
  • Misinzo, G., Meerts, P., Bublot, M., Mast, J., Weingartl, H. M., Nauwynck, H. J. (2005). Binding and entry characteristics of porcine circovirus 2 in cells of the porcine monocytic line 3D4/31. J. Gen. Virol. 86: 2057-2068 [Abstract] [Full Text]  
  • Krey, T., Thiel, H.-J., Rumenapf, T. (2005). Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry. J. Virol. 79: 4191-4200 [Abstract] [Full Text]  
  • Elphick, G. F., Querbes, W., Jordan, J. A., Gee, G. V., Eash, S., Manley, K., Dugan, A., Stanifer, M., Bhatnagar, A., Kroeze, W. K., Roth, B. L., Atwood, W. J. (2004). The Human Polyomavirus, JCV, Uses Serotonin Receptors to Infect Cells. Science 306: 1380-1383 [Abstract] [Full Text]  
  • Eash, S., Querbes, W., Atwood, W. J. (2004). Infection of Vero Cells by BK Virus Is Dependent on Caveolae. J. Virol. 78: 11583-11590 [Abstract] [Full Text]  
  • Chu, J. J. H., Ng, M. L. (2004). Infectious Entry of West Nile Virus Occurs through a Clathrin-Mediated Endocytic Pathway. J. Virol. 78: 10543-10555 [Abstract] [Full Text]  
  • Querbes, W., Benmerah, A., Tosoni, D., Di Fiore, P. P., Atwood, W. J. (2004). A JC Virus-Induced Signal Is Required for Infection of Glial Cells by a Clathrin- and eps15-Dependent Pathway. J. Virol. 78: 250-256 [Abstract] [Full Text]  
  • Akula, S. M., Naranatt, P. P., Walia, N.-S., Wang, F.-Z., Fegley, B., Chandran, B. (2003). Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Infection of Human Fibroblast Cells Occurs through Endocytosis. J. Virol. 77: 7978-7990 [Abstract] [Full Text]  
  • Kim, J., Woolridge, S., Biffi, R., Borghi, E., Lassak, A., Ferrante, P., Amini, S., Khalili, K., Safak, M. (2003). Members of the AP-1 Family, c-Jun and c-Fos, Functionally Interact with JC Virus Early Regulatory Protein Large T Antigen. J. Virol. 77: 5241-5252 [Abstract] [Full Text]  
  • Snyers, L., Zwickl, H., Blaas, D. (2003). Human Rhinovirus Type 2 Is Internalized by Clathrin-Mediated Endocytosis. J. Virol. 77: 5360-5369 [Abstract] [Full Text]  
  • Bousarghin, L., Touze, A., Sizaret, P.-Y., Coursaget, P. (2003). Human Papillomavirus Types 16, 31, and 58 Use Different Endocytosis Pathways To Enter Cells. J. Virol. 77: 3846-3850 [Abstract] [Full Text]  
  • Gilbert, J. M., Goldberg, I. G., Benjamin, T. L. (2003). Cell Penetration and Trafficking of Polyomavirus. J. Virol. 77: 2615-2622 [Abstract] [Full Text]  
  • Mannova, P., Forstova, J. (2003). Mouse Polyomavirus Utilizes Recycling Endosomes for a Traffic Pathway Independent of COPI Vesicle Transport. J. Virol. 77: 1672-1681 [Abstract] [Full Text]  
  • Ashok, A., Atwood, W. J. (2002). Contrasting Roles of Endosomal pH and the Cytoskeleton in Infection of Human Glial Cells by JC Virus and Simian Virus 40. J. Virol. 77: 1347-1356 [Abstract] [Full Text]  
  • Diaz-Griffero, F., Hoschander, S. A., Brojatsch, J. (2002). Endocytosis Is a Critical Step in Entry of Subgroup B Avian Leukosis Viruses. J. Virol. 76: 12866-12876 [Abstract] [Full Text]  
  • Komagome, R., Sawa, H., Suzuki, T., Suzuki, Y., Tanaka, S., Atwood, W. J., Nagashima, K. (2002). Oligosaccharides as Receptors for JC Virus. J. Virol. 76: 12992-13000 [Abstract] [Full Text]  
  • Sieczkarski, S. B., Whittaker, G. R. (2002). Influenza Virus Can Enter and Infect Cells in the Absence of Clathrin-Mediated Endocytosis. J. Virol. 76: 10455-10464 [Abstract] [Full Text]  
  • Stuart, A. D., Eustace, H. E., McKee, T. A., Brown, T. D. K. (2002). A Novel Cell Entry Pathway for a DAF-Using Human Enterovirus Is Dependent on Lipid Rafts. J. Virol. 76: 9307-9322 [Abstract] [Full Text]  
  • Sieczkarski, S. B., Whittaker, G. R. (2002). Dissecting virus entry via endocytosis. J. Gen. Virol. 83: 1535-1545 [Abstract] [Full Text]  
  • Empig, C. J., Goldsmith, M. A. (2002). Association of the Caveola Vesicular System with Cellular Entry by Filoviruses. J. Virol. 76: 5266-5270 [Abstract] [Full Text]  
  • Christensen, N. D., Reed, C. A., Culp, T. D., Hermonat, P. L., Howett, M. K., Anderson, R. A., Zaneveld, L. J. D. (2001). Papillomavirus Microbicidal Activities of High-Molecular-Weight Cellulose Sulfate, Dextran Sulfate, and Polystyrene Sulfonate. Antimicrob. Agents Chemother. 45: 3427-3432 [Abstract] [Full Text]  
  • Richterova, Z., Liebl, D., Horak, M., Palkova, Z., Hozak, P., Korb, J., Forstova, J. (2001). Caveolae Are Involved in the Trafficking of Mouse Polyomavirus Virions and Artificial VP1 Pseudocapsids toward Cell Nuclei. J. Virol. 75: 10880-10891 [Abstract] [Full Text]  
  • Petrogiannis-Haliotis, T., Sakoulas, G., Kirby, J., Koralnik, I. J., Dvorak, A. M., Monahan-Earley, R., De Girolami, P. C., De Girolami, U., Upton, M., Major, E. O., Pfister, L.-A., Joseph, J. T. (2001). BK-Related Polyomavirus Vasculopathy in a Renal-Transplant Recipient. NEJM 345: 1250-1255 [Full Text]  
  • Joki-Korpela, P., Marjomäki, V., Krogerus, C., Heino, J., Hyypiä, T. (2001). Entry of Human Parechovirus 1. J. Virol. 75: 1958-1967 [Abstract] [Full Text]  
  • Gilbert, J. M., Benjamin, T. L. (2000). Early Steps of Polyomavirus Entry into Cells. J. Virol. 74: 8582-8588 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»