This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chiou, P. P.
Right arrow Articles by Leong, J.-A. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chiou, P. P.
Right arrow Articles by Leong, J.-A. C.

 Previous Article  |  Next Article 

Journal of Virology, August 2000, p. 7619-7627, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Infectious Hematopoietic Necrosis Virus Matrix Protein Inhibits Host-Directed Gene Expression and Induces Morphological Changes of Apoptosis in Cell Cultures†

Pinwen P. Chiou,Dagger Carol H. Kim,§ Patricia Ormonde, and Jo-Ann C. Leong*

Department of Microbiology and Center for Salmon Disease Research, Oregon State University, Corvallis, Oregon 97331-3804

Received 6 January 2000/Accepted 24 April 2000

Infectious hematopoietic necrosis virus (IHNV) infection in tissue culture cells has previously been shown to result in the shutdown of host protein synthesis, cell rounding, and cell death. We report here an investigation of the cytopathogenicity of the viral phosphoprotein (P or M1), matrix (M or M2), and nonvirion (NV) proteins in cultured fish cells. The expression of M alone potently inhibited reporter gene expression from a viral and an interferon (IFN)-inducible promoter, whereas P and NV did not produce a similar effect. Northern blot analysis further revealed a reduction in the steady-state level of reporter mRNA when the M gene was cotransfected into cells; conversely, M mRNA was not drastically reduced in the same cells. By immunofluorescence confocal microscopy, fragmented nuclei were found in some cells expressing M protein but not in cells expressing P, NV, or beta -galactosidase protein. Electron microscopy revealed the morphological changes associated with apoptosis in the M-transfected cells. Furthermore, IHNV infection was shown to produce DNA "laddering" in cultured cells. Taken together, these data suggested at least two functions for M protein in an IHNV infection: down regulation of host transcription and the induction of programmed cell death. In the course of these experiments, we also discovered that NV expression was associated with cell rounding, the first biological effect on cells to be attributed to the NV gene.

* Corresponding author. Mailing address: Department of Microbiology, North Hall 220, Oregon State University, Corvallis, OR 97331-3804. Phone: (541) 737-1834. Fax: (541) 737-0496. E-mail: leongj{at}orst.edu.

dagger Oregon Agricultural Experiment Station technical paper 11,687.

Dagger Present address: Biotechnology Center, University of Connecticut, Storrs, CT 06269-3149.

§ Present address: Department of Biochemistry, Microbiology, and Molecular Biology, University of Maine, Orono, ME 04469.

Journal of Virology, August 2000, p. 7619-7627, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kassis, R., Larrous, F., Estaquier, J., Bourhy, H. (2004). Lyssavirus Matrix Protein Induces Apoptosis by a TRAIL-Dependent Mechanism Involving Caspase-8 Activation. J. Virol. 78: 6543-6555 [Abstract] [Full Text]  
  • Alonso, M., Kim, C. H., Johnson, M. C., Pressley, M., Leong, J.-A. (2004). The NV Gene of Snakehead Rhabdovirus (SHRV) Is Not Required for Pathogenesis, and a Heterologous Glycoprotein Can Be Incorporated into the SHRV Envelope. J. Virol. 78: 5875-5882 [Abstract] [Full Text]  
  • Thoulouze, M.-I., Bouguyon, E., Carpentier, C., Bremont, M. (2004). Essential Role of the NV Protein of Novirhabdovirus for Pathogenicity in Rainbow Trout. J. Virol. 78: 4098-4107 [Abstract] [Full Text]  
  • Posavad, C. M., Huang, M. L., Barcy, S., Koelle, D. M., Corey, L. (2000). Long Term Persistence of Herpes Simplex Virus-Specific CD8+ CTL in Persons with Frequently Recurring Genital Herpes. J. Immunol. 165: 1146-1152 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»