This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Akari, H.
Right arrow Articles by Adachi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Akari, H.
Right arrow Articles by Adachi, A.

 Previous Article  |  Next Article 

Journal of Virology, May 2000, p. 4891-4893, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cell-Dependent Requirement of Human Immunodeficiency Virus Type 1 gp41 Cytoplasmic Tail for Env Incorporation into Virions

Hirofumi Akari, Tomoharu Fukumori, and Akio Adachi*

Department of Virology, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan

Received 8 November 1999/Accepted 16 February 2000

Growth kinetics in lymphocytic H9 and M8166 cells of two mutants of human immunodeficiency virus type 1 (HIV-1) with deleted gp41 cytoplasmic tails were examined. While the mutant viruses designated CTdel-44 and CTdel-144 were able to grow in M8166 cells, they were unable to grow in H9 cells. Transfection and single-round infectivity assays demonstrated that they are defective in the early phase of viral replication in H9 cells. Analysis of the mutant virions revealed drastically reduced incorporation of Env gp120 (compared with the incorporation of wild-type virions) in H9 cells but normal incorporation in M8166 cells. These results indicate that the HIV-1 cytoplasmic tail of gp41 determines virus infectivity in a cell-dependent manner by affecting incorporation of Env into virions and suggest the involvement of a host cell factor(s) in the Env incorporation.

* Corresponding author. Mailing address: Department of Virology, The University of Tokushima School of Medicine, 3 Kuramoto, Tokushima 770-8503, Japan. Phone: 81-88-633-7078. Fax: 81-88-633-7080. E-mail: adachi{at}basic.med.tokushima-u.ac.jp.

Journal of Virology, May 2000, p. 4891-4893, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chen, P., Hubner, W., Spinelli, M. A., Chen, B. K. (2007). Predominant Mode of Human Immunodeficiency Virus Transfer between T Cells Is Mediated by Sustained Env-Dependent Neutralization-Resistant Virological Synapses. J. Virol. 81: 12582-12595 [Abstract] [Full Text]  
  • Waheed, A. A., Ablan, S. D., Roser, J. D., Sowder, R. C., Schaffner, C. P., Chertova, E., Freed, E. O. (2007). HIV-1 escape from the entry-inhibiting effects of a cholesterol-binding compound via cleavage of gp41 by the viral protease. Proc. Natl. Acad. Sci. USA 104: 8467-8471 [Abstract] [Full Text]  
  • Lambele, M., Labrosse, B., Roch, E., Moreau, A., Verrier, B., Barin, F., Roingeard, P., Mammano, F., Brand, D. (2007). Impact of Natural Polymorphism within the gp41 Cytoplasmic Tail of Human Immunodeficiency Virus Type 1 on the Intracellular Distribution of Envelope Glycoproteins and Viral Assembly. J. Virol. 81: 125-140 [Abstract] [Full Text]  
  • Lopez-Verges, S., Camus, G., Blot, G., Beauvoir, R., Benarous, R., Berlioz-Torrent, C. (2006). Tail-interacting protein TIP47 is a connector between Gag and Env and is required for Env incorporation into HIV-1 virions. Proc. Natl. Acad. Sci. USA 103: 14947-14952 [Abstract] [Full Text]  
  • Davis, M. R., Jiang, J., Zhou, J., Freed, E. O., Aiken, C. (2006). A Mutation in the Human Immunodeficiency Virus Type 1 Gag Protein Destabilizes the Interaction of the Envelope Protein Subunits gp120 and gp41. J. Virol. 80: 2405-2417 [Abstract] [Full Text]  
  • Yuste, E., Johnson, W., Pavlakis, G. N., Desrosiers, R. C. (2005). Virion Envelope Content, Infectivity, and Neutralization Sensitivity of Simian Immunodeficiency Virus. J. Virol. 79: 12455-12463 [Abstract] [Full Text]  
  • Dave, R. S., Pomerantz, R. J. (2004). Antiviral Effects of Human Immunodeficiency Virus Type 1-Specific Small Interfering RNAs against Targets Conserved in Select Neurotropic Viral Strains. J. Virol. 78: 13687-13696 [Abstract] [Full Text]  
  • Day, J. R., Munk, C., Guatelli, J. C. (2004). The Membrane-Proximal Tyrosine-Based Sorting Signal of Human Immunodeficiency Virus Type 1 gp41 Is Required for Optimal Viral Infectivity. J. Virol. 78: 1069-1079 [Abstract] [Full Text]  
  • Fujita, M., Sakurai, A., Yoshida, A., Miyaura, M., Koyama, A. H., Sakai, K., Adachi, A. (2002). Amino Acid Residues 88 and 89 in the Central Hydrophilic Region of Human Immunodeficiency Virus Type 1 Vif Are Critical for Viral Infectivity by Enhancing the Steady-State Expression of Vif. J. Virol. 77: 1626-1632 [Abstract] [Full Text]  
  • Serhan, F., Jourdan, N., Saleun, S., Moullier, P., Duisit, G. (2002). Characterization of Producer Cell-Dependent Restriction of Murine Leukemia Virus Replication. J. Virol. 76: 6609-6617 [Abstract] [Full Text]  
  • Lee, S.-F., Ko, C.-Y., Wang, C.-T., Chen, S. S.-L. (2002). Effect of Point Mutations in the N Terminus of the Lentivirus Lytic Peptide-1 Sequence of Human Immunodeficiency Virus Type 1 Transmembrane Protein gp41 on Env Stability. J. Biol. Chem. 277: 15363-15375 [Abstract] [Full Text]  
  • Evans, D. T., Tillman, K. C., Desrosiers, R. C. (2002). Envelope Glycoprotein Cytoplasmic Domains from Diverse Lentiviruses Interact with the Prenylated Rab Acceptor. J. Virol. 76: 327-337 [Abstract] [Full Text]  
  • Chen, B. K., Rousso, I., Shim, S., Kim, P. S. (2001). Efficient assembly of an HIV-1/MLV Gag-chimeric virus in murine cells. Proc. Natl. Acad. Sci. USA 10.1073/pnas.261563198v1 [Abstract] [Full Text]  
  • Fujita, M., Yoshida, A., Miyaura, M., Sakurai, A., Akari, H., Koyama, A. H., Adachi, A. (2001). Cyclophilin A-Independent Replication of a Human Immunodeficiency Virus Type 1 Isolate Carrying a Small Portion of the Simian Immunodeficiency Virus SIVMAC gag Capsid Region. J. Virol. 75: 10527-10531 [Abstract] [Full Text]  
  • West, J. T., Johnston, P. B., Dubay, S. R., Hunter, E. (2001). Mutations within the Putative Membrane-Spanning Domain of the Simian Immunodeficiency Virus Transmembrane Glycoprotein Define the Minimal Requirements for Fusion, Incorporation, and Infectivity. J. Virol. 75: 9601-9612 [Abstract] [Full Text]  
  • Chen, S. S.-L., Lee, S.-F., Wang, C.-T. (2001). Cellular Membrane-Binding Ability of the C-Terminal Cytoplasmic Domain of Human Immunodeficiency Virus Type 1 Envelope Transmembrane Protein gp41. J. Virol. 75: 9925-9938 [Abstract] [Full Text]  
  • Miller, E. D., Duus, K. M., Townsend, M., Yi, Y., Collman, R., Reitz, M., Su, L. (2001). Human Immunodeficiency Virus Type 1 IIIB Selected for Replication In Vivo Exhibits Increased Envelope Glycoproteins in Virions without Alteration in Coreceptor Usage: Separation of In Vivo Replication from Macrophage Tropism. J. Virol. 75: 8498-8506 [Abstract] [Full Text]  
  • Zhu, P., Olson, W. C., Roux, K. H. (2001). Structural Flexibility and Functional Valence of CD4-IgG2 (PRO 542): Potential for Cross-Linking Human Immunodeficiency Virus Type 1 Envelope Spikes. J. Virol. 75: 6682-6686 [Abstract] [Full Text]  
  • Chen, B. K., Rousso, I., Shim, S., Kim, P. S. (2001). Efficient assembly of an HIV-1/MLV Gag-chimeric virus in murine cells. Proc. Natl. Acad. Sci. USA 98: 15239-15244 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»