This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hemmings, D. G.
Right arrow Articles by Guilbert, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hemmings, D. G.
Right arrow Articles by Guilbert, L. J.

 Previous Article  |  Next Article 

J Virol, June 1998, p. 4970-4979, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Permissive Cytomegalovirus Infection of Primary Villous Term and First Trimester Trophoblasts

D. G. Hemmings, R. Kilani, C. Nykiforuk, J. Preiksaitis, and L. J. Guilbert*

Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7

Received 4 December 1997/Accepted 2 March 1998

Forty percent of women with primary cytomegalovirus (CMV) infections during pregnancy infect their fetuses with complications for the baby varying from mild to severe. How CMV crosses the syncytiotrophoblast, the barrier between maternal blood and fetal tissue in the villous placenta, is unknown. Virus may cross by infection of maternal cells that pass through physical breaches in the syncytiotrophoblast or by direct infection of the syncytiotrophoblast, with subsequent transmission to underlying fetal placental cells. In this study, we show that pure (>99.99%), long-term and healthy (>3 weeks) cultures of syncytiotrophoblasts are permissively infected with CMV. Greater than 99% of infectious progeny virus remained cell associated throughout culture periods up to 3 weeks. Infection of term trophoblasts required a higher virus inoculum, was less efficient, and progressed more slowly than parallel infections of placental and human embryonic lung fibroblasts. Three laboratory strains (AD169, Towne, and Davis) and a clinical isolate from a congenitally infected infant all permissively infected trophoblasts, although infection efficiencies varied. The infection of first trimester syncytiotrophoblasts with strain AD169 occurred at higher frequency and progressed more rapidly than infection of term cells but less efficiently and rapidly than infection of fibroblasts. These results show that villous syncytiotrophoblasts can be permissively infected by CMV but that the infection requires high virus titers and proceeds slowly and that progeny virus remains predominantly cell associated.

* Corresponding author. Mailing address: HMRC 6-25, University of Alberta, Edmonton, Alberta, Canada T6G 2H7. Phone: (403) 492-4910. Fax: (403) 492-0368. E-mail: larry.guilbert{at}ualberta.ca.

J Virol, June 1998, p. 4970-4979, Vol. 72, No. 6
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nassetta, L., Kimberlin, D., Whitley, R. (2009). Treatment of congenital cytomegalovirus infection: implications for future therapeutic strategies. J Antimicrob Chemother 63: 862-867 [Abstract] [Full Text]  
  • Maidji, E., Genbacev, O., Chang, H.-T., Pereira, L. (2007). Developmental Regulation of Human Cytomegalovirus Receptors in Cytotrophoblasts Correlates with Distinct Replication Sites in the Placenta. J. Virol. 81: 4701-4712 [Abstract] [Full Text]  
  • Chan, G., Stinski, M.F., Guilbert, L.J. (2004). Human Cytomegalovirus-Induced Upregulation of Intercellular Cell Adhesion Molecule-1 on Villous Syncytiotrophoblasts. Biol. Reprod. 71: 797-803 [Abstract] [Full Text]  
  • Yamamoto-Tabata, T., McDonagh, S., Chang, H.-T., Fisher, S., Pereira, L. (2004). Human Cytomegalovirus Interleukin-10 Downregulates Metalloproteinase Activity and Impairs Endothelial Cell Migration and Placental Cytotrophoblast Invasiveness In Vitro. J. Virol. 78: 2831-2840 [Abstract] [Full Text]  
  • Terauchi, M., Koi, H., Hayano, C., Toyama-Sorimachi, N., Karasuyama, H., Yamanashi, Y., Aso, T., Shirakata, M. (2003). Placental Extravillous Cytotrophoblasts Persistently Express Class I Major Histocompatibility Complex Molecules after Human Cytomegalovirus Infection. J. Virol. 77: 8187-8195 [Abstract] [Full Text]  
  • Lazzarotto, T., Gabrielli, L., Foschini, M. P., Lanari, M., Guerra, B., Eusebi, V., Landini, M. P. (2003). Congenital Cytomegalovirus Infection in Twin Pregnancies: Viral Load in the Amniotic Fluid and Pregnancy Outcome. Pediatrics 112: e153-157 [Abstract] [Full Text]  
  • Lenfant, F., Pizzato, N., Liang, S., Davrinche, C., Le Bouteiller, P., Horuzsko, A. (2003). Induction of HLA-G-restricted human cytomegalovirus pp65 (UL83)-specific cytotoxic T lymphocytes in HLA-G transgenic mice. J. Gen. Virol. 84: 307-317 [Abstract] [Full Text]  
  • Revello, M. G., Gerna, G. (2002). Diagnosis and Management of Human Cytomegalovirus Infection in the Mother, Fetus, and Newborn Infant. Clin. Microbiol. Rev. 15: 680-715 [Abstract] [Full Text]  
  • Benz, C., Reusch, U., Muranyi, W., Brune, W., Atalay, R., Hengel, H. (2001). Efficient downregulation of major histocompatibility complex class I molecules in human epithelial cells infected with cytomegalovirus. J. Gen. Virol. 82: 2061-2070 [Abstract] [Full Text]  
  • Fisher, S., Genbacev, O., Maidji, E., Pereira, L. (2000). Human Cytomegalovirus Infection of Placental Cytotrophoblasts In Vitro and In Utero: Implications for Transmission and Pathogenesis. J. Virol. 74: 6808-6820 [Abstract] [Full Text]  
  • British Andrology Society, (1999). British Andrology Society guidelines for the screening of semen donors for donor insemination (1999). Hum Reprod 14: 1823-1826 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»